Fisheries Infosite

FAR 2015/39 New qPCR (quantitative polymerase chain reaction) and haematological sampling methods for monitoring Bonamia exitiosa infections and oyster (Ostrea chilensis) mortality in the Foveaux Strait fishery (OYU 5).

Filename
FAR_2015_39_2729_OYS2011-01 Objectives 2; Milestone 4.pdf

Link to file
FAR_2015_39_2729_OYS2011-01 Objectives 2; Milestone 4.pdf (1.0 MB)

Abstract
A new qPCR method to detect Bonamia exitiosa in oysters from Foveaux Strait comprises a duplex qPCR assay for the co-amplification of the Bonamia target and the Ostrea chilensis β-actin gene, shortened lab procedures, and a block to allow effective co-amplification. This method has greater sensitivity and specificity than heart imprints (performance coefficient of 0.73 when compared to qPCR heart samples and 0.61 for gill samples), and is more cost-effective, but requires further development.

Document date
Friday, 4 September 2015
Document type
V 1.3
File format
Adobe PDF
File size
1.0 MB
Reference number
2015/39
Sort order


Uploaded from
FAR_2015_39_2729_OYS2011-01 Objectives 2; Milestone 4.pdf

Uploaded date
Friday, 4 September 2015

Search tags
FAR: 2015/39;
ISSN: 1179-5352;
ISBN: 978-0-908334-95-7;
ScienceStock: Shellfish;
Species: Bonamia exitiosa; Ostrea chilensis; Oysters;
Stock: OYU5;
ResearchField: Bonamia exitiosa; Ostrea chilensis; qPCR;
Author: Forman; Hulston; Maas; Mackay; McVeagh; Michael;

If you are having problems opening this file, you may not have the software on your computer needed to view it. Click the link below to download and install a suitable program.