FAR 2015/39 New qPCR (quantitative polymerase chain reaction) and haematological sampling methods for monitoring Bonamia exitiosa infections and oyster (Ostrea chilensis) mortality in the Foveaux Strait fishery (OYU 5).

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FAR_2015_39_2729_OYS2011-01 Objectives 2; Milestone 4.pdf

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FAR_2015_39_2729_OYS2011-01 Objectives 2; Milestone 4.pdf (1.0 MB)

Abstract
A new qPCR method to detect Bonamia exitiosa in oysters from Foveaux Strait comprises a duplex qPCR assay for the co-amplification of the Bonamia target and the Ostrea chilensis β-actin gene, shortened lab procedures, and a block to allow effective co-amplification. This method has greater sensitivity and specificity than heart imprints (performance coefficient of 0.73 when compared to qPCR heart samples and 0.61 for gill samples), and is more cost-effective, but requires further development.

Document date
Friday, 4 September 2015

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V 1.3

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Adobe PDF

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1.0 MB

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2015/39

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FAR_2015_39_2729_OYS2011-01 Objectives 2; Milestone 4.pdf

Uploaded date
Friday, 4 September 2015

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FAR: 2015/39;
ISSN: 1179-5352;
ISBN: 978-0-908334-95-7;
ScienceStock: Shellfish;
Species: Bonamia exitiosa; Ostrea chilensis; Oysters;
Stock: OYU5;
ResearchField: Bonamia exitiosa; Ostrea chilensis; qPCR;
Author: Forman; Hulston; Maas; Mackay; McVeagh; Michael;

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