Fisheries Infosite

FAR 2015/39 New qPCR (quantitative polymerase chain reaction) and haematological sampling methods for monitoring Bonamia exitiosa infections and oyster (Ostrea chilensis) mortality in the Foveaux Strait fishery (OYU 5).

Filename
FAR_2015_39_2729_OYS2011-01 Objectives 2; Milestone 4.pdf

Link to file
FAR_2015_39_2729_OYS2011-01 Objectives 2; Milestone 4.pdf (1.0 MB)

Abstract
A new qPCR method to detect Bonamia exitiosa in oysters from Foveaux Strait comprises a duplex qPCR assay for the co-amplification of the Bonamia target and the Ostrea chilensis β-actin gene, shortened lab procedures, and a block to allow effective co-amplification. This method has greater sensitivity and specificity than heart imprints (performance coefficient of 0.73 when compared to qPCR heart samples and 0.61 for gill samples), and is more cost-effective, but requires further development.

Document date
Friday, 4 September 2015
Document type
V 1.3
File format
Adobe PDF
File size
1.0 MB
Reference number
978-0-908334-95-7
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Uploaded from
FAR_2015_39_2729_OYS2011-01 Objectives 2; Milestone 4.pdf

Uploaded date
Friday, 4 September 2015

Search tags
Author: Forman, J.; Hulston, D.; Maas, E.; Mackay, J.; McVeagh, M.; Michael, K.;
ResearchField: Bonamia exitiosa; Ostrea chilensis; qPCR;
ScienceStock: Shellfish;
Species: Bonamia exitiosa; Ostrea chilensis; Oysters;
Stock: OYU5;

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